jonathon pines (Addgene inc)
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jonathon pines
Jonathon Pines, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jonathon+pines/pCerulean-N1+Cyclin+B1+(1147)+(Plasmid+%2361852)/bio_rxiv__64898__2026__01__27__700572-154-9-11
Average 94 stars, based on 1 article reviews
Jonathon Pines, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jonathon+pines/pCerulean-N1+Cyclin+B1+(1147)+(Plasmid+%2361852)/bio_rxiv__64898__2026__01__27__700572-154-9-11
Average 94 stars, based on 1 article reviews
jonathon pines - by Bioz Stars,
2026-09
94/100 stars
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In Vitro:Article Title: SGK1 is essential for meiotic resumption in mammalian oocytes. Article Snippet: Images were assembled in software LAS X (Leica Microsystems) and signal intensity from the spindle area was quantified with ImageJ. .. Cdk1-AF and H2b:gfp RNAs were in vitro transcribed by using the correspondent plasmid templates (Cdk1-AF: pcDNA3-CDC2-AF (718) was a gift from Plasmid Preparation:Article Title: SGK1 is essential for meiotic resumption in mammalian oocytes. Article Snippet: Images were assembled in software LAS X (Leica Microsystems) and signal intensity from the spindle area was quantified with ImageJ. .. Cdk1-AF and H2b:gfp RNAs were in vitro transcribed by using the correspondent plasmid templates (Cdk1-AF: pcDNA3-CDC2-AF (718) was a gift from Article Title: Live dynamics of induced cell-cell fusion between mitotic and interphasic cells Article Snippet: .. The pCerulean-N1 Cyclin B1 (1147) was a gift from Article Title: SGK1 is essential for meiotic resumption in mammalian oocytes. Article Snippet: .. The plasmid containing Cdk1-AF (pcDNA3-cdc2-AF (718)) was a gift from Article Title: Multiple intersecting pathways are involved in CPEB1 phosphorylation and regulation of translation during mouse oocyte meiosis. Article Snippet: .. The CDK1 FRET sensor was a gift from Article Title: Phosphorylation of the DNA repair scaffold SLX4 drives folding of the SAP domain and activation of the MUS81-EME1 endonuclease. Article Snippet: Plasmid pENTR221 SLX4resist M1A was constructed by replacing the BamHI-XbaI (NEB) fragment of pENTR221 SLX4 (a kind gift from Stephen West) with a partially complementary sequence (Bio Basic Inc.) containing the following non-coding substitutions: c.1020G>T, c.1027G>A, c.1029T>A, c.1032T>C, c.1035C>T, c.1038A>G, c.2490G>T, c.2493G>A, c.2496T>C, c.2499G>A, c.2502G>A, and c.2505A>G. .. Plasmid pDEST FRT/TO N-Venus-3XFLAGGateway was constructed from pDEST FRT/TO N-FLAG, a kind gift from Dr. Stephen West, and pcDNA5 FRT/TO Venus-FLAGGateway (1124), a gift from Article Title: Master mitotic kinases regulate viral genome delivery during papillomavirus cell entry. Article Snippet: HPV16 pL2-EGFP SSTP212AAAA, -S212A, -S213A, -T214A, -P215A, BPV1 pL2-EGFP TSTP239AAAA, -T241A, HPV18 pL2EGFP SSTP211AAAA, -T213A, and HPV5 pL2-EGFP T249A were generated from pL2-EGFP by site-directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies) according to manufacturer’s instruction. .. For immunoprecipitations, HPV16 pL2-3xHA SSTP212AAAA, S212A, S213A, T214A, and P215A were generated from pL2-3xHA WT4 with mutagenic primers by using the QuikChange II XL Site-DirectedMutagenesis Kit (Agilent Technologies) according to manufacturer’s instruction. pcDNA3-Plk1(829) was a gift from Article Title: Multitask Approach to Localize Rhizobial Type Three Secretion System Effector Proteins Inside Eukaryotic Cells. Article Snippet: .. Acknowledgments: pcDNA5/FRT/TO-Venus-Flag (865) was a gift from Control:Article Title: SGK1 is essential for meiotic resumption in mammalian oocytes. Article Snippet: Images were assembled in software LAS X (Leica Microsystems) and signal intensity from the spindle area was quantified with ImageJ. .. Cdk1-AF and H2b:gfp RNAs were in vitro transcribed by using the correspondent plasmid templates (Cdk1-AF: pcDNA3-CDC2-AF (718) was a gift from Article Title: SGK1 is essential for meiotic resumption in mammalian oocytes. Article Snippet: .. The plasmid containing Cdk1-AF (pcDNA3-cdc2-AF (718)) was a gift from Construct:Article Title: Phosphorylation of the DNA repair scaffold SLX4 drives folding of the SAP domain and activation of the MUS81-EME1 endonuclease. Article Snippet: Plasmid pENTR221 SLX4resist M1A was constructed by replacing the BamHI-XbaI (NEB) fragment of pENTR221 SLX4 (a kind gift from Stephen West) with a partially complementary sequence (Bio Basic Inc.) containing the following non-coding substitutions: c.1020G>T, c.1027G>A, c.1029T>A, c.1032T>C, c.1035C>T, c.1038A>G, c.2490G>T, c.2493G>A, c.2496T>C, c.2499G>A, c.2502G>A, and c.2505A>G. .. Plasmid pDEST FRT/TO N-Venus-3XFLAGGateway was constructed from pDEST FRT/TO N-FLAG, a kind gift from Dr. Stephen West, and pcDNA5 FRT/TO Venus-FLAGGateway (1124), a gift from Article Title: Dual-localized PPTC7 limits mitophagy through proximal and dynamic interactions with BNIP3 and NIX. Article Snippet: .. Positive constructs were incubated with LR clonase and the pcDNA5/FRT/TO-Venus-Flag-Gateway destination vector, which was a kind gift from Generated:Article Title: Master mitotic kinases regulate viral genome delivery during papillomavirus cell entry. Article Snippet: HPV16 pL2-EGFP SSTP212AAAA, -S212A, -S213A, -T214A, -P215A, BPV1 pL2-EGFP TSTP239AAAA, -T241A, HPV18 pL2EGFP SSTP211AAAA, -T213A, and HPV5 pL2-EGFP T249A were generated from pL2-EGFP by site-directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies) according to manufacturer’s instruction. .. For immunoprecipitations, HPV16 pL2-3xHA SSTP212AAAA, S212A, S213A, T214A, and P215A were generated from pL2-3xHA WT4 with mutagenic primers by using the QuikChange II XL Site-DirectedMutagenesis Kit (Agilent Technologies) according to manufacturer’s instruction. pcDNA3-Plk1(829) was a gift from Incubation:Article Title: Dual-localized PPTC7 limits mitophagy through proximal and dynamic interactions with BNIP3 and NIX. Article Snippet: .. Positive constructs were incubated with LR clonase and the pcDNA5/FRT/TO-Venus-Flag-Gateway destination vector, which was a kind gift from |